By default, NT is optimised for a 256k L2 cache, and will set HKLM\SYSTEM\CurrentControlSet\Control\Session Manager\MemoryManagement\SecondLevelDataCache to 0. If this key doesn't exist, it simply means that NT is using the default.
If your processor has a larger L2 cache, it is worth adding the full value, as a REG_DWORD. You can enter the value as decimal (512, 1024 or however much L2 cache you have).
It won't make the machine fly, but you may notice a slight improvement in response times. Intel processors are particularly inefficient at utilising the L2 cache anyway.
I hope this helps