Yes. I assume that you are using VB with an Access MDB file. If you create the MDB file in Access 2000, use the database utilities to save it as prior Access version. Use a data control and in the Connect property of the data control specify "Access" not "Access 2000".
Actually, Access should not even need to be installed on the users machine. Both Access and VB use the Jet database engine native. If you author the MDB file in Access 2000 and set the Connect property of any data controls to Access 2000 it should work.