Assuming no alteration to the BIOS booting order, and the disk is partitioned, formatted and has an op system on it, then the answer is YES. I assume you're planning to make a fresh installation of W2K PRO on this new drive, or maybe do a disk-to-disk copy, and ditch the existing drive.
Re the cable, I think it is usual for the Master to be placed at the end, but having said that, I've connected the Slave at the end and Master in the middle and never had any problems.
Going on from that, would anyone care to tell me what the maximum data cable length is for these ATA100 80-wire ribbons?
ROGER - GØAOZ.