Whenever you change something in an LPAR's profile, you either have to to that change again via the DLPAR menu on the HMC to make that change visible to that LPAR or you have to shutdown/activate the LPAR.
Simply rebooting the LPAR has no effect, because it uses the LPAR-profile info from the last activation.
Required/Desired setting for an I/O slot can only be changed in the LPAR profile, not via DLPAR operation (I think), so in this case: shutdown/activate profile at next convenient maintenance timeslot.
HTH,
p5wizard